Anti-CES1 Polyclonal Antibody is a Rabbit antibody targeting CES1. Anti-CES1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Pig,Cow,Rabbit)

1. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CES1 Polyclonal Antibody, Unconjugated (TMAB-00418) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CES1/ Liver Carboxylesterase 1 Polyclonal Antibody, Unconjugated (TMAB-00418) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Protein: intestinal (mouse) lysate at 40 μg;
Primary: rabbit Anti-CES1/Liver Carboxylesterase 1 (TMAB-00418) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000;
Predicted band size: 61 kDa
Observed band size: 66 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CES1

Human

Lipoprotein metabolism,Cholesterol Metabolism,Hydrolysis,Lipoproteins/Apolipoproteins,Drug metabolism,Cholesterol Metabolism,Hydrolysis,Lipoprotein metabolism,Cancer,Heart disease,Drug metabolism,Lipid metabolism