Anti-CES1 Polyclonal Antibody is a Rabbit antibody targeting CES1. Anti-CES1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Pig,Cow,Rabbit)

1. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CES1 Polyclonal Antibody, Unconjugated (TMAB-00418) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CES1/ Liver Carboxylesterase 1 Polyclonal Antibody, Unconjugated (TMAB-00418) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Protein: intestinal (mouse) lysate at 40 μg;
Primary: rabbit Anti-CES1/Liver Carboxylesterase 1 (TMAB-00418) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000;
Predicted band size: 61 kDa
Observed band size: 66 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CES1

Human

Ces1

Liver carboxylesterase 1

Lipoprotein metabolism,Cholesterol Metabolism,Hydrolysis,Lipoproteins/Apolipoproteins,Drug metabolism,Cholesterol Metabolism,Hydrolysis,Lipoprotein metabolism,Cancer,Heart disease,Drug metabolism,Lipid metabolism

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.