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Anti-CD47 Antibody (1F304) 是一种抗体,靶向 CD47。Anti-CD47 Antibody (1F304) 可用于 ELISA, FC。
Anti-CD47 Antibody (1F304) 是一种抗体,靶向 CD47。Anti-CD47 Antibody (1F304) 可用于 ELISA, FC。
产品描述 | Anti-CD47 Antibody (1F304) is an antibody targeting CD47. Anti-CD47 Antibody (1F304) can be used in ELISA, FC. |
别名 | OA3, MER6, IAP, CD47 molecule |
Ig Type | hIgG1 |
克隆号 | 1F304 |
交叉反应 | Human |
验证活性 | 1. The Binding Activity of Human CD47 with Anti-CD47 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 μg/mL can bind Anti-CD47 recombinant antibody. The EC50 is 1.343-1.561 ng/mL. 2. Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-CD47 antibody (TMAH-00206) (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry. 3. Untransfected CT26 cells (green line) and transfected Human DLL3 & CD47 CT26 stable cells (red line) were stained with anti-DLL3 antibody (2µg/1*10^6 cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry. |
应用 | ELISA, FC |
推荐剂量 | FC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
研究背景 | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transd... |
偶联 | Unconjugated |
免疫原 | Recombinant Human CD47 protein |
抗原种属 | Human |
基因ID | 961 |
Uniprot ID | |
研究领域 | Cancer, Immunology |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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