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Anti-CD45 Polyclonal Antibody
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产品编号 TMAB-00373
Anti-CD45 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD45。Anti-CD45 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-CD45 Polyclonal Antibody
Anti-CD45 Polyclonal Antibody
还可以产品编号 TMAB-00373
Anti-CD45 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD45。Anti-CD45 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-CD45 Polyclonal Antibody is a Rabbit antibody targeting CD45. Anti-CD45 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. 25 μg total protein per Lane of various lysates probed with CD45 polyclonal antibody, unconjugated (TMAB-00373) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-800; IHC-Fr: 1:100-800; ICC/IF: 1:50-200; IF: 1:100-800 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling |
| 免疫原 | KLH conjugated synthetic peptide: human CD45 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Endothelial Cell Markers,CD,Regulatory T Cells,CD,Non-lineage,Microglia marker,Tyrosine Phosphatases,B Lymphocytic Lineage,NK Cell Lineage,T Lymphocytic Lineage,Dendritic Cell Lineage,Mast Cell Lineage,Monocytic Lineage,Neutrophil Lineage,Surface Molecules |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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