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Anti-Cathepsin K Polyclonal Antibody

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Anti-Cathepsin K Polyclonal Antibody
产品编号 TMAB-00298

Anti-Cathepsin K Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Cathepsin K。Anti-Cathepsin K Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-Cathepsin K Polyclonal Antibody

Anti-Cathepsin K Polyclonal Antibody

Rating icon 还可以
Anti-Cathepsin K Polyclonal Antibody
产品编号 TMAB-00298

Anti-Cathepsin K Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Cathepsin K。Anti-Cathepsin K Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-Cathepsin K Polyclonal Antibody is a Rabbit antibody targeting Cathepsin K. Anti-Cathepsin K Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,GuineaPig)
验证活性
1. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Cathepsin K Polyclonal Antibody, Unconjugated (TMAB-00298) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Mouse 4T1 cell lysates
Primary: Anti-Cathepsin K (TMAB-00298) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 46 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Lysosome.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
KLH conjugated synthetic peptide: human Cathepsin K
抗原种属
Human
基因ID
Uniprot ID
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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