Anti-Caspase-1 p20 Polyclonal Antibody 2 is a Rabbit antibody targeting Caspase-1 p20. Anti-Caspase-1 p20 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Sample:
Stomach (Mouse) Lysate at 40 μg
Stomach (Rat) Lysate at 40 μg
Large intestine (Rat) Lysate at 40 μg
Primary: Anti-Caspase-1 p20 (TMAB-00283) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/46 kDa
Observed band size: 20 kDa
2. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (TMAB-00283)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Caspase-1 p20) Polyclonal Antibody, Unconjugated (TMAB-00283) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Caspase-1 p20

Human

Response to hypoxia,Caspases,Metabolism,Caspases,Caspases,Apoptosis,Hypoxia