Anti-BMP-7 Polyclonal Antibody is a Rabbit antibody targeting BMP-7. Anti-BMP-7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat,Rabbit (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Sample:
293T Cell (Human) Lysate at 30 μg
Primary: Anti-BMP7 (TMAB-00245) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15.7/47 kDa
Observed band size: 49/40 kDa
2. Tissue/cell: rabbit alveolar bone; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BMP7 Polyclonal Antibody, Unconjugated (TMAB-00245) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Bone (Mouse) Lysate at 40 μg
Primary: Anti-BMP7 (TMAB-00245) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15.7/47 kDa
Observed band size: 47,50 kDa
4. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Placenta (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Mouse) Lysate at 40 μg
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-BMP7 (TMAB-00245) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BMP7

Human

Cell differentiation,Cell differentiation,Kidney development,Nervous system development,Lipid metabolism,Bone,TGF,Secreted