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Anti-Beclin-1 Antibody (3S337)
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还可以产品编号 TMAH-00114
Anti-Beclin-1 Antibody (3S337) 是一种抗体，靶向 Beclin-1。Anti-Beclin-1 Antibody (3S337) 可用于 ELISA,FCM。
Anti-Beclin-1 Antibody (3S337)
Anti-Beclin-1 Antibody (3S337)
一键复制产品信息 还可以产品编号 TMAH-00114
Anti-Beclin-1 Antibody (3S337) 是一种抗体，靶向 Beclin-1。Anti-Beclin-1 Antibody (3S337) 可用于 ELISA,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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生物活性
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抗原信息
| 产品描述 | Anti-Beclin-1 Antibody (3S337) is an antibody targeting Beclin-1. Anti-Beclin-1 Antibody (3S337) can be used in ELISA,FCM. |
| Ig Type | Rabbit IgG |
| 克隆号 | 3S337 |
| 交叉反应 | Human |
| 验证活性 | Overlay Peak curve showing RAJI cells stained with TMAH-00114 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISAFCM |
| 推荐剂量 | FCM:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome. Cytoplasmic vesicle, autophagosome.; [Beclin-1-C 35 kDa]: Mitochondrion. Nucleus. Cytoplasm.; [Beclin-1-C 37 kDa]: Mitochondrion. |
| 组织特异性 | Ubiquitous. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human BECN1 |
| 抗原种属 | Human |
| 基因名称 | BECN1 |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Metabolism, Microbiology, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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