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Anti-ARNT Antibody (1Z917)
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产品编号 TMAH-00082
Anti-ARNT Antibody (1Z917) 是一种抗体，靶向 ARNT。Anti-ARNT Antibody (1Z917) 可用于 ELISA, WB, IHC, IP。
Anti-ARNT Antibody (1Z917)
Anti-ARNT Antibody (1Z917)
还可以产品编号 TMAH-00082
Anti-ARNT Antibody (1Z917) 是一种抗体，靶向 ARNT。Anti-ARNT Antibody (1Z917) 可用于 ELISA, WB, IHC, IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ARNT Antibody (1Z917) is an antibody targeting ARNT. Anti-ARNT Antibody (1Z917) can be used in ELISA, WB, IHC, IP. |
| Ig Type | Rabbit IgG |
| 克隆号 | 1Z917 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate -All lanes: ARNT antibody at 1.83μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 87, 85, 86 KDa -Observed band size: 87 KDa 2. IHC image of TMAH-00082 diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunoprecipitating HIF-1 beta in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00082 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00082 (3μg) + Hela whole cell lysate (500μg) -Lane 3: Hela whole cell lysate (20μg) |
| 应用 | |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human ARNT |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cardiovascular |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
SCI 文献
关键词
Hypoxia-inducible factor 1-β (HIF-1-β;HIF1-β)Hypoxia-inducible factor 1-beta (HIF-1-beta;HIF1-beta)Hypoxia-inducible factor 1-b (HIF-1-b;HIF1-b)HIF1-βHIF-1-βHIF1-betaHIF-1-betaHIF1-bHIF-1-bDioxin receptor, nuclear translocatorClass E basic helix-loop-helix protein 2 (bHLHe2)BHLHE2bHLHe 2Aryl hydrocarbon receptor nuclear translocatorARNT proteinARNT
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