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Anti-Argonaute-2 Polyclonal Antibody
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产品编号 TMAB-00146
Anti-Argonaute-2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Argonaute-2。Anti-Argonaute-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Argonaute-2 Polyclonal Antibody
Anti-Argonaute-2 Polyclonal Antibody
还可以产品编号 TMAB-00146
Anti-Argonaute-2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Argonaute-2。Anti-Argonaute-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Argonaute-2 Polyclonal Antibody is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AGO2) Polyclonal Antibody, Unconjugated (TMAB-00146) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-eIF2C2 (TMAB-00146) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa 3. Sample: Uterus (Mouse) Lysate at 40 μg Primary: Anti-eIF2C2 (TMAB-00146) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5). |
| 免疫原 | KLH conjugated synthetic peptide: human AGO2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | RNAi,Nuclear Pore Complex,Argonaute and Piwi,Eukaryotic Initiation factors (eIF's) |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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