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Anti-Arginase 1/ARG1 Polyclonal Antibody
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产品编号 TMAB-00144
Anti-Arginase 1/ARG1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Arginase 1/ARG1。Anti-Arginase 1/ARG1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Arginase 1/ARG1 Polyclonal Antibody
Anti-Arginase 1/ARG1 Polyclonal Antibody
还可以产品编号 TMAB-00144
Anti-Arginase 1/ARG1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Arginase 1/ARG1。Anti-Arginase 1/ARG1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Arginase 1/ARG1 Polyclonal Antibody is a Rabbit antibody targeting Arginase 1/ARG1. Anti-Arginase 1/ARG1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Rat (predicted:Mouse,Dog,Pig,Rabbit) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Arginase 1) Polyclonal Antibody, Unconjugated (TMAB-00144) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Arginase 1 (TMAB-00144) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 34 kDa 3. Sample: Liver (Rat) Lysate at 40 μg Primary: Anti-Arginase 1 (TMAB-00144) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 34 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Arginase I which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea . The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence. |
| 免疫原 | KLH conjugated synthetic peptide: human Arginase 1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Amino acid metabolism,Amino Acids |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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