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Anti-4EBP1 Polyclonal Antibody
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产品编号 TMAB-00004
Anti-4EBP1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 4EBP1。Anti-4EBP1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-4EBP1 Polyclonal Antibody
Anti-4EBP1 Polyclonal Antibody
还可以产品编号 TMAB-00004
Anti-4EBP1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 4EBP1。Anti-4EBP1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-4EBP1 Polyclonal Antibody is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Cow,Horse,Goat,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with eIF4EBP1 Polyclonal Antibody, Unconjugated (TMAB-00004) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-eIF4EBP1 (TMAB-00004) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kDa Observed band size: 17 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]. |
| 免疫原 | KLH conjugated synthetic peptide: human 4E-BP1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | RNAi,Regulation,Eukaryotic Initiation factors (eIF's),Diabetes,Metabolic disorders,Obesity |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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