Anti-4EBP1 Polyclonal Antibody is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Horse,Goat,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with eIF4EBP1 Polyclonal Antibody, Unconjugated (TMAB-00004) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-eIF4EBP1 (TMAB-00004) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 17 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human 4E-BP1

Human

EIF4EBP1

Eukaryotic translation initiation factor 4E-binding protein 1

RNAi,Regulation,Eukaryotic Initiation factors (eIF's),Diabetes,Metabolic disorders,Obesity

Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.