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alpha-Amanitin

产品编号 T13540 CAS 23109-05-9

别名: α-Amanitin, α-Amatoxin

alpha-Amanitin is the principal toxin of poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.

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alpha-Amanitin, CAS 23109-05-9

规格	价格/CNY		货期	数量
1 mg	￥ 3,970		35日内发货

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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述	alpha-Amanitin is the principal toxin of poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.
体外活性	Alpha-Amanitin decreases TAF15 mRNA and TAF15 protein levels in MKN45 cells and inhibits the RNAPII activity towards TAF15 mRNA [2]. alpha-Amanitin decreases cell viability by 14%, 21%, 41%, 44%, and 50% at concentrations of 100, 10, 1, 0.1, and 0.01 μg/mL, respectively. The LD50 of the alpha-Amanitin at 36 h is measured as 1 μg/mL. The total amount of protein within the cell at 24 h is significantly increased for the 1 μg/mL dose of alpha-Amanitin compared to the control [3]. Alpha-Amanitin dramatically decreases the expression of gap junctional genes and FSHr and LHR in cumulus cells [4].
体内活性	The intravenous LD50 dose of alpha-Amanitin is 0.327 mg/kg body weight after intravenous injection into BALB/c mice. After 12 h of alpha-Amanitin injection in the caudal vein, the levels of WBC, RBC, and HGB decrease significantly, while those of BUN and Crea increase significantly in serum. alpha-Amanitin inhibits some genes (Irx4, Hsp90b1, etc.), whose encoded proteins regulate the RNA polymerase II activity. alpha-Amanitin down-regulates some proteins (Nmi, Trpc5, etc.) taking part in the transcription progress [1]. alpha-Amanitin has potent activity in DTC suppression. Mice injected with alpha-Amanitin (0.4?mg/kg, i.p.)-treated cells maintain their body weight, while those receiving a peritoneal injection of MKN45 cells show a constant decrease in body weight [2].

别名	α-Amanitin, α-Amatoxin
分子量	918.97
分子式	C39H54N10O14S
CAS No.	23109-05-9

存储

store at low temperature　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 33.33 mg/mL (36.27 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
H2O	1 mM	1.0882 mL	5.4409 mL	10.8817 mL	27.2044 mL
	5 mM	0.2176 mL	1.0882 mL	2.1763 mL	5.4409 mL
	10 mM	0.1088 mL	0.5441 mL	1.0882 mL	2.7204 mL
	20 mM	0.0544 mL	0.272 mL	0.5441 mL	1.3602 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

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参考文献

1. Zhao J, et al. Pathological effects of the mushroom toxin alpha-amanitin on BALB/c mice. Peptides. 2006 Dec;27(12):3047-3052. 2. Kume K, et al. α-Amanitin Restrains Cancer Relapse from Drug-Tolerant Cell Subpopulations via TAF15. Sci Rep. 2016 May 16;6:25895 3. Kaya E, et al. Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line. Turk J Med Sci. 2014;44(5):728-32 4. Park MW, et al. RNA Polymerase II Inhibitor, α-Amanitin, Affects Gene Expression for Gap Junctions and Metabolic Capabilities of Cumulus Cells, but Not Oocyte, during in vitro Mouse Oocyte Maturation. Dev Reprod. 2013 Mar;17(1):63-72

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

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第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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