Ac-DMLD-CMK TFA is a peptide designed to target the caspase3-Gsdme signaling pathway in mice. It specifically inhibits the activation of caspase 3 and Gsdme, preventing the cleavage of Gsdme by caspase 3, thus inhibiting pyroptosis. This action alleviates damage to renal tubular epithelial cells and reduces the secretion of inflammatory cytokines. Ac-DMLD-CMK TFA also helps to lower serum creatinine and blood urea nitrogen levels in mice induced by Cisplatin, mitigating renal function deterioration. It holds potential for studying nephrotoxicity caused by chemotherapeutic agents.