Powder: -20°C for 3 years | In solvent: -80°C for 1 year
20-HETE (20-hydroxy Arachidonic Acid) is a CYP450 metabolite and a potent vasoconstrictor and it is an endogenous inhibitor of the large-conductance Ca2+-activated K+ channel in renal arterioles. 20-HETE increases NADPH oxidase, ROS, and NF-κB activity, and it also inhibits endothelial NO synthase and inhibits apoptosis of pulmonary artery smooth muscle cells[1][2]. 20-HETE constricts smooth muscles, stimulates smooth muscle proliferation and migration.
产品描述 | 20-HETE (20-hydroxy Arachidonic Acid) is a CYP450 metabolite and a potent vasoconstrictor and it is an endogenous inhibitor of the large-conductance Ca2+-activated K+ channel in renal arterioles. 20-HETE increases NADPH oxidase, ROS, and NF-κB activity, and it also inhibits endothelial NO synthase and inhibits apoptosis of pulmonary artery smooth muscle cells[1][2]. 20-HETE constricts smooth muscles, stimulates smooth muscle proliferation and migration. |
体外活性 | 20-HETE promotes platelet-derived growth factor-stimulated vascular smooth muscle cell migration via pathways that involve MEK and phosphatidylinositol 3-kinase activation[2]. 20-HETE induces the phosphorylation of ERK1/2, a MAPK that plays a pivotal role in the proliferation induced by the activation of receptor tyrosine kinases and G protein-coupled receptors. 20-HETE (1-1000 nM) reduces the diameter of isolated perfused small renal arteries of the rat by approximately 15% tetraethylammonium (1 mM) blocked the vasoconstrictor response to 20-HETE (100 nM)[1]. Addition of 20-HETE to the bath (1-100 nM), reduces the frequency of opening of the large-conductance Ca2+-activated K+ channel recorded using cell-attached patches on vascular smooth muscle cells (VSM)[1]. |
体内活性 | In Sprague-Dawley rats, administration of the 20-HETE inhibitor HET0016 or the 20-HETE antagonist 20-HEDE preventes DHT-induced increases in blood pressure as well as abrogates DHT-induced increases in the media-to-lumen ratio (M/L), media thickness, and collagen IV deposition in renal interlobar arteries. 20-HETE is a key regulator of microvascular remodeling in hypertension; its effect is independent of blood pressure elevation and androgen levels[2]. 20-HETE contributes to DHT-induced vascular remodeling independent of blood pressure elevation[2]. In Cyp4a14-/- mice, which display androgen-driven and 20-HETE-dependent hypertension, treatment with the 20-HETE antagonist abolishes remodeling of renal resistance arteries measured as media thickness and M/L. |
别名 | 20-hydroxy Arachidonic Acid |
分子量 | 320.47 |
分子式 | C20H32O3 |
CAS No. | 79551-86-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 3.2 mg/mL (9.99 mM)
Ethanol: 6.67 mg/mL (20.81 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.1204 mL | 15.6021 mL | 31.2042 mL | 78.0104 mL |
5 mM | 0.6241 mL | 3.1204 mL | 6.2408 mL | 15.6021 mL | |
Ethanol | 10 mM | 0.312 mL | 1.5602 mL | 3.1204 mL | 7.801 mL |
20 mM | 0.156 mL | 0.7801 mL | 1.5602 mL | 3.9005 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
20-HETE 79551-86-3 Others 20-hydroxy Arachidonic Acid 20 HETE 20HETE Inhibitor inhibitor inhibit