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顶部生物活性储存和溶解信息计算器

2-Carboxybenzaldehyde

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产品编号 TN6514Cas号 119-67-5

The major peak of 2-Carboxybenzaldehyde reductase activity in human liver co-purifies with hAFAR protein. Sulphydryl substances and some proteins play important roles in preserving the molecular and catalytic properties of 2-Carboxybenzaldehyde reductase.

2-Carboxybenzaldehyde

2-Carboxybenzaldehyde

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产品编号 TN6514Cas号 119-67-5

The major peak of 2-Carboxybenzaldehyde reductase activity in human liver co-purifies with hAFAR protein. Sulphydryl substances and some proteins play important roles in preserving the molecular and catalytic properties of 2-Carboxybenzaldehyde reductase.

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25 g
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The major peak of 2-Carboxybenzaldehyde reductase activity in human liver co-purifies with hAFAR protein. Sulphydryl substances and some proteins play important roles in preserving the molecular and catalytic properties of 2-Carboxybenzaldehyde reductase.
体外活性
  1. A rat liver cytosol enzyme, tentatively named CBA reductase, catalyses the conversion of 2-Carboxybenzaldehyde (CBA) to 2-hydroxymethyl benzoic acid in the presence of NADH (or NADPH). CBA reductase is useful for exploring the mechanism of in vitro enzyme induction, as the enzyme can be induced by phenobarbital (PB) both in vivo and in vitro. METHODS AND RESULTS:2. Possible involvement of glutathione (GSH) in gene expression was suggested by a recent study with cultured rat hepatocytes. 3. CBA reductase was purified about 200-fold by a combination of column chromatography and isoelectric focusing in the presence of mercaptoethanol. 4. The ability to form 2-hydroxymethyl benzoic acid was lost when the enzyme was chromatographed on a hydroxylapatite column in the absence of mercaptoethanol; however, it was restored if sulphydryl compounds or bovine serum albumin was added to the eluate from the column. 5. Gel filtration showed the molecular sizes of CBA reductase from the 105,000g supernatant fraction of rat liver extracts and the purified preparation were 64 kDa and 49 kDa, respectively. CONCLUSIONS:6. The results suggest that sulphydryl substances and some proteins play important roles in preserving the molecular and catalytic properties of CBA reductase.
化学信息
分子量150.13
分子式C8H6O3
CAS No.119-67-5
密度1.4
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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关键词

2-Carboxybenzaldehyde2Carboxybenzaldehyde2 Carboxybenzaldehyde

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