Leucyl-leucyl-leucine is may be used for pH-dependent endosomal membrane disruption to release agents. Trileucine is used to evaluate the absorption characteristics of reversed phase liquid chromatography (HPLC) media.
Leu-AMS, a leucine analogue and potent inhibitor of leucyl-tRNA synthetase (LRS) with an IC50 of 22.34 nM, exhibits cytotoxic effects in both cancer and normal cells and hampers bacterial growth. It specifically inhibits LRS's catalytic activity without impacting the leucine-induced activation of mTORC1.