fam-deala-hyp-yipd

FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (Kd= 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF, also available.

VHL Ligand 38 (Compound 1) 是一种E3 泛素连接酶VHL的竞争性抑制剂，在1%和10% DMSO 溶液中的IC50值分别为4.1 μM和7.0 μM。该化合物包含羟脯氨酸残基和异噁唑乙酰胺片段，能够显著抑制源自HIF1α的荧光肽 (FAM-DEALA-Hyp-YIPD)与VHL的结合。

FAM-DEALAHypYIPMDDDFQLRSF is a 5,6-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (KD= 3 nM). Can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD.