5,6-dehydroArachidonicacid is an analog of arachidonicacid with an acetylene in the 5,6 position. It inhibits 5-LO in rat basophilic leukemia cells, with a Ki value of 15 μM. In guinea pig leukocytes, 5-LO is inhibited by 5,6-dehydroarachidonicacid with an IC50 value of 10 μM.