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VEGF165 Protein, Human/Cynomolgus, Recombinant is expressed in Baculovirus Insect Cells expression system. The predicted molecular weight is 19.2 kDa and the accession number is P15692-4.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μg | ¥ 1,950 | 5日内发货 | |
100 μg | ¥ 4,920 | 现货 | |
500 μg | ¥ 12,740 | 5日内发货 |
生物活性 | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 3-12 ng/mL. |
产品描述 | VEGF165 Protein, Human/Cynomolgus, Recombinant is expressed in Baculovirus Insect Cells expression system. The predicted molecular weight is 19.2 kDa and the accession number is P15692-4. |
种属 | Human, Cynomolgus |
表达系统 | Baculovirus Insect Cells |
标签 | Tag Free |
蛋白编号 | P15692-4 |
别名 | VPF,VEGFA,VEGF,Vascular permeability factor,L-VEGF |
蛋白构建 | A DNA sequence encoding the human / cynomolgus VEGF165 isoform (P15692-4) (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical. |
蛋白纯度 | > 97 % as determined by SDS-PAGE |
分子量 | 19.2 kDa (predicted); 20 and 22 kDa (reducing conditions, corresponding to the monomer) |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Lyophilized from sterile 100mM Glycine, 10mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor. |
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