VEGF164 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 19.4 kDa and the accession number is Q00731-2.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 750 | 现货 | |
| 10 μg | ¥ 1,220 | 5日内发货 | |
| 20 μg | ¥ 1,950 | 5日内发货 | |
| 50 μg | ¥ 3,680 | 5日内发货 | |
| 100 μg | ¥ 5,930 | 5日内发货 | |
| 200 μg | ¥ 9,590 | 5日内发货 | |
| 500 μg | ¥ 18,060 | 5日内发货 |
| 生物活性 | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-22 ng/mL.  |
| 产品描述 | VEGF164 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 19.4 kDa and the accession number is Q00731-2. |
| 种属 | Mouse |
| 表达系统 | Baculovirus Insect Cells |
| 标签 | Tag Free |
| 蛋白编号 | Q00731-2 |
| 别名 | 血管内皮生长因子,Vpf,Vegf,vascular endothelial growth factor A |
| 蛋白构建 | A DNA sequence encoding the mouse VEGF164 (isoform VEGF-1) (Q00731-2) (Met 1-Arg 190) was expressed and purified. Predicted N terminal: Ala 27 |
| 蛋白纯度 | ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.   |
| 分子量 | 19.4 kDa (predicted); 24 kDa (reducing conditions) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, pH7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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