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VEGF121 Protein, Human, Recombinant (E. coli)

产品编号 TMPS-00168

VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9.

VEGF121 Protein, Human, Recombinant (E. coli)

产品编号 TMPS-00168

VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9.

规格	价格	库存	数量
10 μg	¥ 1,080	5日内发货
50 μg	¥ 3,960	5日内发货

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产品信息

生物活性	ED 50 < 5.0 ng/ml, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2.0 × 10 5 units/mg.
产品描述	VEGF121 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P15692-9.
种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	P15692-9
别名	VPF,VEGF-121,Vascular Permeability Factor,Vascular Endothelial Growth Factor 121
蛋白构建	Pro28-Arg147
蛋白纯度	> 95% as determined by SDS-PAGE; > 95% as determined by HPLC
分子量	~28.2 kDa (Non-reducing conditions)
内毒素	< 0.2 EU/μg of protein as determined by the LAL method.
缓冲液	Lyophilized from a 0.2 μm filtered solution in PBS.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development.