规格

价格

库存

数量

5 μg

¥ 213

现货

10 μg

¥ 313

现货

20 μg

¥ 469

3日内发货

50 μg

¥ 833

3日内发货

100 μg

¥ 1,280

现货

200 μg

¥ 1,980

5日内发货

500 μg

¥ 3,780

5日内发货

生物活性

产品描述	TNF alpha Trimer Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P01375.
生物活性	Immobilized Human TNF alpha, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 2.7 ng/ml determined by ELISA (QC Test). Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL (QC Test).
研究背景	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
种属	Human
表达系统	HEK293 Cells
标签	Tag Free
蛋白编号	P01375
蛋白构建	Val77-Leu233
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名	肿瘤坏死因子, Tumor Necrosis Factor Ligand Superfamily Member 2, tumor necrosis factor, Tumor Necrosis Factor, TNF-α, TNFα, TNFSF-2, TNFSF2, TNFSF1A, TNFSF 2, TNFATNF, TNF-alpha, TNF-Alpha, TNFalpha, TNF-a, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein
内毒素	< 0.1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	17.35 kDa (Predicted); 18-22 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

TNF alpha Trimer Protein, Human, Recombinant

TNF alpha Trimer Protein, Human, Recombinant

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