生物活性

产品描述	TL1A/TNFSF15 Trimer Protein, Human, Recombinant (R32A & R85A, His & Flag) is expressed in HEK293 Cells. The accession number is O95150.
生物活性	Immobilized Human TNFSF15 (R32A, R85A) Trimer, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA (QC Test).
研究背景	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
种属	Human
表达系统	HEK293 Cells
标签	N-His-Flag
蛋白编号	O95150
蛋白构建	Asp91-Leu251 trimer design
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 0.1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	58.15 kDa (Predicted); 65-75 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

TL1A/TNFSF15 Trimer Protein, Human, Recombinant (R32A & R85A, His & Flag)

TL1A/TNFSF15 Trimer Protein, Human, Recombinant (R32A & R85A, His & Flag)

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