生物活性

产品描述	Tissue Factor Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P13726-1.
生物活性	Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test).
研究背景	Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P13726-1
蛋白构建	Ser33-Glu251
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	27.7 kDa (Predicted); 35-50 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

Tissue Factor Protein, Human, Recombinant (His & Avi), Biotinylated

Tissue Factor Protein, Human, Recombinant (His & Avi), Biotinylated

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