TEV Protease Protein, TEV, Recombinant (His)

产品编号 TMPS-00131

Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG.

The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6×His tag, allowing for convenient removal after digestion via Ni²⁺ affinity chromatography.

The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.

TEV Protease Protein, TEV, Recombinant (His)

产品编号 TMPS-00131

Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG.<br/><br/>The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6×His tag, allowing for convenient removal after digestion via Ni²⁺ affinity chromatography.<br/><br/>The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.

规格	价格	库存	数量

大包装 & 定制

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

实验操作小课堂

常见问题解答

资源下载

重组蛋白用户指南

产品信息

生物活性	10 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.
产品描述	Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG. The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6×His tag, allowing for convenient removal after digestion via Ni²⁺ affinity chromatography. The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.
标签	His
蛋白纯度	> 95% as determined by SDS-PAGE
分子量	28.4 kDa (Reducing conditions)
缓冲液	Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.
存储	Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG. The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates.

SCI 文献

计算器

复溶计算器
重组蛋白稀释计算器
比活力计算器

技术支持

请阅读重组蛋白用户指南了解更多具体信息.