Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 980 | 5日内发货 | ||
50 μg | ¥ 2,960 | 5日内发货 | ||
500 μg | ¥ 14,800 | 5日内发货 | ||
1 mg | ¥ 22,200 | 5日内发货 |
产品描述 | Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
种属 | Human |
表达系统 | E. coli |
标签 | N-6xHis |
蛋白编号 | P04053 |
别名 | Terminal transferase, Terminal deoxynucleotidyltr, DNTT, Terminal deoxynucleotidyltransferase, DNA nucleotidylexotransferase, TDT, Terminal addition enzyme |
氨基酸序列 | Met1-Ala509 |
蛋白构建 | Met1-Ala509 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 62 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Supplied as a 0.2 μm filtered solution of 0.1M KH<sub>2</sub>PO<sub>4</sub>, 200 mM KCl, 1 mM 2-Mercaptoethanol, 50% Glycerol, pH 7.2. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
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TDT Protein, Human, Recombinant (His) Terminal transferase Terminal deoxynucleotidyltr DNTT Terminal deoxynucleotidyltransferase DNA nucleotidylexotransferase TDT Terminal addition enzyme recombinant recombinant-proteins proteins protein