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SUMO protease, also known as Ulp, is a highly specific enzyme that removes small ubiquitin-like modifier (SUMO) from recombinant SUMO fusion proteins. Unlike proteases such as enterokinase or TEV, which recognize linear amino acid sequences, SUMO protease recognizes the native tertiary structure of SUMO, allowing precise cleavage without leaving extra residues at the cleavage site.
This recombinant SUMO protease is derived from Saccharomyces cerevisiae, expressed in E. coli, and purified to obtain high yields of active enzyme under animal-free conditions. It is well suited for applications in drug and vaccine development, protein manufacturing, and other research uses. A C-terminal 6×His tag is included to enable easy removal of the protease following cleavage via Ni²⁺ affinity purification.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
250 U | ¥ 720 | 5日内发货 | |
1000 U | ¥ 2,160 | 5日内发货 |
生物活性 | 10 U/μl Unit definition: One
unit of SUMO Protease cleaves ≥ 85% of 2 μg control substrate
in 1 h at 30℃ |
产品描述 | SUMO protease, also known as Ulp, is a highly specific enzyme that removes small ubiquitin-like modifier (SUMO) from recombinant SUMO fusion proteins. Unlike proteases such as enterokinase or TEV, which recognize linear amino acid sequences, SUMO protease recognizes the native tertiary structure of SUMO, allowing precise cleavage without leaving extra residues at the cleavage site.
This recombinant SUMO protease is derived from Saccharomyces cerevisiae, expressed in E. coli, and purified to obtain high yields of active enzyme under animal-free conditions. It is well suited for applications in drug and vaccine development, protein manufacturing, and other research uses. A C-terminal 6×His tag is included to enable easy removal of the protease following cleavage via Ni²⁺ affinity purification. |
种属 | Saccharomyces
cerevisiae |
标签 | His |
蛋白纯度 | ≥ 95% as determined by SDS-PAGE |
分子量 | 27 kDa (Predicted) |
内毒素 | < 0.2 EU/μg of protein as determined by the LAL method. |
存储 | Upon receiving, the product remains stable for
up to 6 months at -20 °C. This product is stable for up to 1 week at 37 °C.
Avoid repeated freeze-thaw cycles by making single-use aliquots before the
solution is stored at -20 °C. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | SUMO protease, also known as Ulp, is a highly specific enzyme that removes small ubiquitin-like modifier (SUMO) from recombinant SUMO fusion proteins. Unlike proteases such as enterokinase or TEV, which recognize linear amino acid sequences, SUMO protease recognizes the native tertiary structure of SUMO, allowing precise cleavage without leaving extra residues at the cleavage site. |