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ST6GAL1 Protein, Mouse, Recombinant (His)

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货号 TMPY-02371

别名 St6Gal-I, St6galI, St6gal, ST6 β-galactosamide α-2,6-sialyltranferase 1, ST6 beta-galactosamide α-2,6-sialyltranferase 1, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, Siat1, AW742324

ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.

ST6GAL1 Protein, Mouse, Recombinant (His)
其他形式的 “ST6GAL1 Protein, Mouse, Recombinant (His)”:

ST6GAL1 Protein, Mouse, Recombinant (His)

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货号 TMPY-02371 别名 St6Gal-I, St6galI, St6gal, ST6 β-galactosamide α-2,6-sialyltranferase 1, ST6 beta-galactosamide α-2,6-sialyltranferase 1, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, Siat1, AW742324

ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.

规格价格库存数量
5 μg
¥ 496
6-8日内发货
10 μg
¥ 791
6-8日内发货
20 μg
¥ 1,330
5日内发货
50 μg
¥ 2,650
5日内发货
100 μg
¥ 4,470
5日内发货
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产品介绍


生物活性
产品描述
ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.
生物活性
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is > 150 pmol/min/μg.
研究背景
Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein that belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the Golgi but can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
种属
Mouse
表达系统
HEK293 Cells
标签N-His
蛋白编号Q64685
蛋白构建A DNA sequence encoding the extracellular domain of mouse ST6GAL1 (NP_666045.1) (Lys 27-Cys 403) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: His
蛋白纯度
> 96 % as determined by SDS-PAGE
ST6GAL1 Protein, Mouse, Recombinant (His)
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 120 mM NaCl, pH7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名St6Gal-I, St6galI, St6gal, ST6 β-galactosamide α-2,6-sialyltranferase 1, ST6 beta-galactosamide α-2,6-sialyltranferase 1, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, Siat1, AW742324
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量45.9 kDa (predicted); 50-55 kDa (reducing conditions)
颜色White
物理性状Lyophilized powder
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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剂量转换

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