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SARS-COV-2 Spike S Trimer Protein (D614G, His & Avi)

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货号 TMPK-00435

别名 Spike glycoprotein, S protein, S glycoprotein, COVID-19

The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.

SARS-COV-2 Spike S Trimer Protein (D614G, His & Avi)

SARS-COV-2 Spike S Trimer Protein (D614G, His & Avi)

一键复制产品信息
Rating icon 很棒
SPR兼容缓冲液
货号 TMPK-00435 别名 Spike glycoprotein, S protein, S glycoprotein, COVID-19

The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.

规格价格库存数量
5 μg
¥ 343
3日内发货
10 μg
¥ 549
3日内发货
20 μg
¥ 898
3日内发货
50 μg
¥ 1,760
3日内发货
100 μg
¥ 2,960
现货
200 μg
¥ 5,350
5日内发货
500 μg
¥ 11,800
5日内发货
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纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
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产品介绍


生物活性
产品描述
The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.
生物活性
Immobilized SARS-COV-2 Spike S Trimer (D614G) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.
研究背景
The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.
种属
SARS-CoV-2
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号A0A6G7K2L4
蛋白构建Val16-Glu1188 (D614G)
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名Spike glycoprotein, S protein, S glycoprotein, COVID-19
内毒素< 1 EU/μg by the LAL method.
化学信息
分子量136.7 kDa (predicted). Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

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剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

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