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SIRT5 Protein, Human, Recombinant (His)

产品编号 TMPY-06954

SIRT5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30.78 kDa and the accession number is NP_036373.1.

SIRT5 Protein, Human, Recombinant (His)

SIRT5 Protein, Human, Recombinant (His)

产品编号 TMPY-06954
SIRT5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30.78 kDa and the accession number is NP_036373.1.
规格价格库存数量
100 μg
¥ 4,460
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
超声应使用什么仪器?
建议使用“超声清洗仪”,不推荐用“超声破碎仪”。
溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思?
这句话指的是,如果溶解度 < 1 mg/mL,那我们就认为它是微溶或不溶的,这是针对写的“DMSO:Insoluble”或“DMSO:slightly soluble”这类化合物的。
抑制剂加 DMSO 配成母液之后,能否放在常温的环境保存1周?
不建议这么做,母液配置好分装保存,建议存于 -20℃ 或 -80℃,尽量避免反复冻融。如果您订购的规格很大,可以考虑分装粉末保存,固体粉末是比较稳定的,-20℃ 可以保存三年。
母液配置的计算方法?
(1) c=n/v=m/M/v 浓度=物质的量/体积=质量/相对分子质量/体积 (2) 官网产品详情页下方有溶液配制表格,可以参考计算好的加入溶剂体积
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
SIRT5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30.78 kDa and the accession number is NP_036373.1.
种属
Human
表达系统
E. coli
标签N-His
蛋白编号NP_036373.1
别名
sirtuin 5,SIR2L5
蛋白构建
A DNA sequence encoding the Human SIRT5 (NP_036373.1) (Ser37-Ser310) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
蛋白纯度
≥ 95 % as determined by SDS-PAGE.
分子量30.78 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 10% Glycerol, pH 9. 0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The sirtuin SIRT5 resides primarily in the mitochondrial matrix and catalyzes the removal of negatively charged lysine acyl modifications; succinyl, malonyl, and glutaryl groups SIRT5 as a significant regulator of cellular homeostasis, in a context- and cell-type specific manner, as has been observed previously for other sirtuin family members. SIRT5 regulates protein substrates involved in glycolysis, the TCA cycle, fatty acid oxidation, electron transport chain, ketone body formation, nitrogenous waste management, and ROS detoxification, among other processes. SIRT5 plays pivotal roles in cardiac physiology and stress responses and is involved in the regulation of numerous aspects of myocardial energy metabolism. SIRT5 is implicated in neoplasia, as both a tumor promoter and suppressor in a context-specific manner, and may serve a protective function in the setting of neurodegenerative disorders. The SIRT5 downregulation is associated with increased succinylation and activity of ACOX1 and oxidative DNA damage response in hepatocellular carcinoma (HCC).

参考文献

SCI 文献

计算器

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  • 重组蛋白稀释 计算器
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