Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,700 | 5日内发货 | ||
500 μg | ¥ 14,800 | 5日内发货 | ||
1 mg | ¥ 24,700 | 5日内发货 |
生物活性 | Immobilized Human SIRP alpha V5, His Tag at 1μg/ml (100μl/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 39.1ng/ml determined by ELISA. |
产品描述 | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. |
种属 | Human |
表达系统 | HEK293 |
标签 | C-His-Avi |
蛋白编号 | P78324 variant 5 |
别名 | MFR, PTPNS1, P84, BIT, MYD-1, MYD1, SHPS-1, SIRPA, SIRP α, SIRP α V5, SHPS1, SIRP alpha, CD172a |
蛋白构建 | Recombinant Human SIRP alpha V5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg370(S42F).[Accession |P78324 variant 5] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. |
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SIRP alpha V5 Protein, Human, Recombinant (His & Avi) MFR PTPNS1 P84 BIT SHPS 1 MYD-1 PTPNS-1 PTPNS 1 P-84 MYD1 SHPS-1 SIRPA SIRP α SIRP α V5 P 84 MYD 1 SHPS1 SIRP alpha CD172a recombinant recombinant-proteins proteins protein