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SARS-CoV Nucleocapsid Protein (His)

SARS-CoV Nucleocapsid Protein (His)

产品编号 TMPY-02870
别名: coronavirus Nucleocapsid Protein, SARS, coronavirus NP Protein, SARS, novel coronavirus NP Protein, SARS, ncov NP Protein, SARS, novel coronavirus, cov np Protein, SARS, novel coronavirus Nucleocapsid Protein, SARS, coronavirus Nucleoprotein Protein, SARS

SARS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is Q6S8E1.

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SARS-CoV Nucleocapsid Protein (His)
规格 价格/CNY 货期 数量
100 μg ¥ 5,750 5日内发货
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产品目录号及名称: SARS-CoV Nucleocapsid Protein (His) (TMPY-02870)
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生物活性 Testing in progress
产品描述 SARS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is Q6S8E1.
种属 SARS
表达系统 Baculovirus Insect Cells
标签 C-His
蛋白编号 Q6S8E1
别名 coronavirus Nucleocapsid Protein, SARS, coronavirus NP Protein, SARS, novel coronavirus NP Protein, SARS, ncov NP Protein, SARS, novel coronavirus, cov np Protein, SARS, novel coronavirus Nucleocapsid Protein, SARS, coronavirus Nucleoprotein Protein, SARS
蛋白构建 SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422)
蛋白纯度 > 80 % as determined by SDS-PAGE
分子量 47.5 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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Keywords

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