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RPE65 Protein, Human, Recombinant (His & Myc)

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货号 TMPH-02033

别名 RPE65, Retinol isomerase, Retinoid isomerohydrolase, Retinal pigment epithelium-specific 65 kDa protein, Meso-zeaxanthin isomerase, Lutein isomerase, All-trans-retinyl-palmitate hydrolase

RPE65 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.

RPE65 Protein, Human, Recombinant (His & Myc)
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RPE65 Protein, Human, Recombinant (His & Myc)

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货号 TMPH-02033 别名 RPE65, Retinol isomerase, Retinoid isomerohydrolase, Retinal pigment epithelium-specific 65 kDa protein, Meso-zeaxanthin isomerase, Lutein isomerase, All-trans-retinyl-palmitate hydrolase

RPE65 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.

规格价格库存数量
5 μg
¥ 1,090
20日内发货
10 μg
¥ 1,850
20日内发货
20 μg
¥ 3,120
20日内发货
50 μg
¥ 5,890
20日内发货
100 μg
¥ 9,630
20日内发货
200 μg
¥ 12,200
20日内发货
500 μg
¥ 16,700
20日内发货
1 mg
¥ 21,300
20日内发货
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产品介绍


生物活性
产品描述
RPE65 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.
生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景
Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.
种属
Human
表达系统
Baculovirus Insect Cells
标签N-10xHis, C-Myc
蛋白编号Q16518
氨基酸序列SIQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS
蛋白构建2-533 aa
蛋白纯度
> 85% as determined by SDS-PAGE.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名RPE65, Retinol isomerase, Retinoid isomerohydrolase, Retinal pigment epithelium-specific 65 kDa protein, Meso-zeaxanthin isomerase, Lutein isomerase, All-trans-retinyl-palmitate hydrolase
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量64.7 kDa (predicted)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

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剂量转换

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技术支持

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