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Rotavirus A (strain St. Thomas 3) VP4 Protein (His)

产品编号 TMPH-03425

Rotavirus A (strain St. Thomas 3) VP4 Protein (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 28.3 kDa and the accession number is P11200.

Rotavirus A (strain St. Thomas 3) VP4 Protein (His)

Rotavirus A (strain St. Thomas 3) VP4 Protein (His)

产品编号 TMPH-03425
Rotavirus A (strain St. Thomas 3) VP4 Protein (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 28.3 kDa and the accession number is P11200.
规格价格库存数量
20 μg
¥ 2,570
20日内发货
100 μg
¥ 5,370
20日内发货
500 μg
¥ 12,300
20日内发货
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常见问题解答
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
抑制剂能否用于细胞实验?
可以的,我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
抑制剂产品应如何储存?
粉末储存在 -20℃,可以存 3 年以上。 母液一般储存于 -80℃,可以存 1 年以上,建议多分装几管,避免反复冻融。 常用的存于 4℃,可放一周以上。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Rotavirus A (strain St. Thomas 3) VP4 Protein (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 28.3 kDa and the accession number is P11200.
种属
RV-A
表达系统
P. pastoris (Yeast)
标签N-6xHis
蛋白编号P11200
别名
Outer capsid protein VP4,Hemagglutinin
氨基酸序列
AQVSEDIIISKTSLWKEMQYNRDIIIRFKFNNSIIKLGGLGYKWSEISFKAANYQYNYLRDGEQVTAHTTCSVNGVNNFSYNGGLLPTHFSISRYEVIKENSYVYVDYWDDSQAFRNMVYVRSLAANLNSVKCSGGNYNFQMPVGAWPVMSGGAVSLHFAGVTLSTQFTDFVSLNSLRFRFSLTVEEPPFSILRTRVSGLYGLPASNPNSGHEYYEIAGRFSLISLVPSNDDY
蛋白构建
247-479 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量28.3 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. It is subsequently lost, together with VP7, following virus entry into the host cell. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7. During the virus exit from the host cell, VP4 seems to be required to target the newly formed virions to the host cell lipid rafts.; Forms the spike 'foot' and 'body' and acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. During entry, the part of VP5* that protrudes from the virus folds back on itself and reorganizes from a local dimer to a trimer. This reorganization may be linked to membrane penetration by exposing VP5* hydrophobic region. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.; Forms the head of the spikes and mediates the recognition of specific host cell surface glycans. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact. In some other strains, VP8* mediates the attachment to histo-blood group antigens (HBGAs) for viral entry.

参考文献

SCI 文献

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