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PKC iota Protein, Human, Recombinant (GST)

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产品编号 TMPY-04455U

PKC iota Protein, Human, Recombinant (GST) is expressed in Baculovirus Insect Cells with an N-terminal GST tag. The accession number is AAH22016.3.

PKC iota Protein, Human, Recombinant (GST)

一键复制产品信息

产品编号 TMPY-04455U

PKC iota Protein, Human, Recombinant (GST) is expressed in Baculovirus Insect Cells with an N-terminal GST tag. The accession number is AAH22016.3.

规格	价格	库存
10 μg	待询	待询
50 μg	待询	待询
500 μg	待询	待询

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产品信息

生物活性	The specific activity of PKC iota was determined to be 45 nmol /min/mg as per activity assay protocol.
产品描述	PKC iota Protein, Human, Recombinant (GST) is expressed in Baculovirus Insect Cells with an N-terminal GST tag. The accession number is AAH22016.3.
种属	Human
表达系统	Baculovirus-Insect Cells
标签	N-GST
蛋白编号	AAH22016.3
别名	Protein kinase C iota type,PRKC-lambda/iota,PRKCI,nPKC-iota,DXS1179E,aPKC-lambda/iota
蛋白构建	Recombinant full length human PKC iota was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
蛋白纯度	> 85% as determined by SDS-PAGE
分子量	94 kDa (predicted); 110 kDa (reducing conditions)
蛋白性状	Solution
缓冲液	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, 10-25% glycerol.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	Enzymes are highly recommended to be shipped at frozen temperature with dry ice.
研究背景	Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCI contains one AGC-kinase C-terminal domain, one OPR domain, one phorbol-ester/DAG-type zinc finger and one protein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.