Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. It contains 1 PIPK domain and is expressed ubiquitously, with high levels in the brain. It catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It may exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. It may regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation, negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. It also involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 1,170 | 5日内发货 | ||
50 μg | ¥ 3,470 | 5日内发货 | ||
500 μg | ¥ 12,100 | 5日内发货 | ||
1 mg | ¥ 18,900 | 5日内发货 |
产品描述 | Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. It contains 1 PIPK domain and is expressed ubiquitously, with high levels in the brain. It catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It may exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. It may regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation, negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. It also involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. |
种属 | Human |
表达系统 | Human Cells |
标签 | C-6His |
蛋白编号 | P48426 |
别名 | Diphosphoinositide kinase 2-α, PtdIns(5)P-4-kinase isoform 2-alpha, PIP5KIII, Phosphatidylinositol 5-phosphate 4-kinase type II α, 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, 1-phosphatidylinositol 5-phosphate 4-kinase 2-α, PtdIns(5)P-4-kinase isoform 2-α, PtdIns(4)P-5-kinase B isoform, Diphosphoinositide kinase 2-alpha, PtdIns(4)P-5-kinase C isoform |
氨基酸序列 | Met1-Thr406 |
蛋白构建 | Recombinant Human Phosphatidylinositol 5-phosphate 4-kinase Type-2 Alpha is produced by our Mammalian expression system and the target gene encoding Met1-Thr406 is expressed with a 6His tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 52-58 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. It contains 1 PIPK domain and is expressed ubiquitously, with high levels in the brain. It catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It may exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. It may regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation, negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. It also involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
PIP4K2A Protein, Human, Recombinant (His) Diphosphoinositide kinase 2-α PtdIns(5)P-4-kinase isoform 2-alpha PIP5KIII Phosphatidylinositol 5-phosphate 4-kinase type II α 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha Phosphatidylinositol 5-phosphate 4-kinase type II alpha 1-phosphatidylinositol 5-phosphate 4-kinase 2-α PtdIns(5)P-4-kinase isoform 2-α PtdIns(4)P-5-kinase B isoform Diphosphoinositide kinase 2-alpha PtdIns(4)P-5-kinase C isoform recombinant recombinant-proteins proteins protein