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Phospholipase C Protein, Clostridium perfringens, Recombinant (His)

Phospholipase C Protein, Clostridium perfringens, Recombinant (His)

产品编号 TMPH-00421

Phospholipase C Protein, Clostridium perfringens, Recombinant (His) is expressed in E. coli.

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Phospholipase C Protein, Clostridium perfringens, Recombinant (His)
规格 价格/CNY 货期 数量
20 μg ¥ 2,290 20日内发货
100 μg ¥ 4,320 20日内发货
1 mg ¥ 14,600 20日内发货
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产品目录号及名称: Phospholipase C Protein, Clostridium perfringens, Recombinant (His) (TMPH-00421)
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生物活性
技术参数
产品性质
产品描述 Phospholipase C Protein, Clostridium perfringens, Recombinant (His) is expressed in E. coli.
种属 C.perfringens
表达系统 E. coli
标签 N-6xHis
蛋白编号 P0C216
氨基酸序列 WDGKIDGTGTHAMIVTQGVSILENDLSKNEPESVRKNLEILKENMHELQLGSTYPDYDKNAYDLYQDHFWDPDTDNNFSKDNSWYLAYSIPDTGESQIRKFSALARYEWQRGNYKQATFYLGEAMHYFGDIDTPYHPANVTAVDSAGHVKFETFAEERKEQYKINTAGCKTNEDFYADILKNKDFNAWSKEYARGFAKTGKSIYYSHASMSHSWDDWDYAAKVTLANSQKGTAGYIYRFLHDVSEGNDPSVGKNVKELVAYISTSGEKDAGTDDYMYFGIKTKDGKTQEWEMDNPGNDFMTGSKDTYTFKLKDENLKIDDIQNMWIRKRKYTAFPDAYKPENIKIIANGKVVVDKDINEWISGNSTYNIK
蛋白构建 29-398 aa
蛋白纯度 > 85% as determined by SDS-PAGE.
分子量 48.5 kDa (predicted)
缓冲液 If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.

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Keywords

recombinant recombinant-proteins proteins protein

 

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