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Pectate lyase 1 Protein, Juniperus ashei, Recombinant (His)

产品编号 TMPH-02367

Has low pectate lyase activity. Pectate lyase 1 Protein, Juniperus ashei, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 39.6 kDa and the accession number is P81294.

Pectate lyase 1 Protein, Juniperus ashei, Recombinant (His)

Pectate lyase 1 Protein, Juniperus ashei, Recombinant (His)

产品编号 TMPH-02367
Has low pectate lyase activity. Pectate lyase 1 Protein, Juniperus ashei, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 39.6 kDa and the accession number is P81294.
规格价格库存数量
20 μg
¥ 2,570
20日内发货
100 μg
¥ 5,370
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
超声应使用什么仪器?
建议使用“超声清洗仪”,不推荐用“超声破碎仪”。
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
收到产品发现冰盒都化了,怎么办?
一般化合物粉末对温度不敏感,加冰盒是为了防止极端气温,如果冰盒融化不影响产品质量。
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Has low pectate lyase activity. Pectate lyase 1 Protein, Juniperus ashei, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 39.6 kDa and the accession number is P81294.
种属
Juniperus ashei
表达系统
P. pastoris (Yeast)
标签N-6xHis
蛋白编号P81294
别名
Pectate lyase 1,Major pollen allergen Jun a 1
氨基酸序列
DNPIDSCWRGDSNWDQNRMKLADCAVGFGSSTMGGKGGDFYTVTSTDDNPVNPTPGTLRYGATREKALWIIFSQNMNIKLKMPLYVAGHKTIDGRGADVHLGNGGPCLFMRKVSHVILHSLHIHGCNTSVLGDVLVSESIGVEPVHAQDGDAITMRNVTNAWIDHNSLSDCSDGLIDVTLGSTGITISNNHFFNHHKVMLLGHDDTYDDDKSMKVTVAFNQFGPNAGQRMPRARYGLVHVANNNYDPWNIYAIGGSSNPTILSEGNSFTAPSESYKKEVTKRIGCESPSACANWVWRSTRDAFINGAYFVSSGKTEETNIYNSNEAFKVENGNAAPQLTKNAGVVT
蛋白构建
22-367 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量39.6 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Has low pectate lyase activity.

参考文献

引用文献

SCI 文献

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