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PDGF-BB Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is B1H0W5.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 938 | 6-8日内发货 | |
| 10 μg | ¥ 1,560 | 6-8日内发货 | |
| 20 μg | ¥ 2,530 | 5日内发货 | |
| 50 μg | ¥ 4,830 | 5日内发货 | |
| 100 μg | ¥ 7,920 | 5日内发货 | |
| 200 μg | ¥ 12,700 | 5日内发货 | |
| 500 μg | ¥ 23,800 | 5日内发货 | |
| 1 mg | ¥ 38,800 | 5日内发货 |
| 生物活性 | ED 50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED 50 , the calculated specific activity is approximately > 0.5 × 10 5 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay. |
| 产品描述 | PDGF-BB Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is B1H0W5. |
| 种属 | Bovine |
| 表达系统 | P. pastoris (Yeast) |
| 标签 | Tag Free |
| 蛋白编号 | B1H0W5 |
| 别名 | PDGF-BB,PDGFBB |
| 蛋白构建 | Ser82-Thr190 |
| 蛋白纯度 | ≥ 95% as determined by SDS-PAGE |
| 分子量 | 12 kDa (Predicted); 10-18 kDa (Reducing conditions) |
| 内毒素 | < 0.1 EU/μg of protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a 0.2 μm filtered solution in 20 mM NaAc, pH 5.0. |
| 复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |