生物活性

产品描述	PDGF-AA Protein, Rat, Recombinant (His) is expressed in P. pastoris (Yeast). The accession number is P28576-1.
生物活性	Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5-15 ng/ml.
研究背景	PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements.
种属	Rat
表达系统	P. pastoris (Yeast)
标签	N-His
蛋白编号	P28576-1
蛋白构建	Arg86-Lys204
蛋白纯度	> 90% as determined by Bis-Tris PAGE
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	14.75 kDa (Predicted); 17-22 kDa and 25-45 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

PDGF-AA Protein, Rat, Recombinant (His)

PDGF-AA Protein, Rat, Recombinant (His)

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