生物活性

产品描述	OX40L/TNFSF4 Trimer Protein, Human, Recombinant (His & Flag), Biotinylated is expressed in HEK293 Cells. The accession number is P23510-1.
生物活性	Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8 ng/ml determined by ELISA (QC Test).
研究背景	Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
种属	Human
表达系统	HEK293 Cells
标签	N-His-Flag
蛋白编号	P23510-1
蛋白构建	Gln51-Leu183 trimer design
蛋白纯度	> 90% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	49.7 kDa (Predicted); 65-160 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

OX40L/TNFSF4 Trimer Protein, Human, Recombinant (His & Flag), Biotinylated

OX40L/TNFSF4 Trimer Protein, Human, Recombinant (His & Flag), Biotinylated

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