OPCML Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32.7 kDa and the accession number is Q6DFY2.
生物活性 | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse OPCmL-His at 10μg/mL (100μL/well) can bind human LSAMP-Fc . The EC50 of human LSAMP-Fc is 0.04-0.1 μg/mL. |
产品描述 | OPCML Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32.7 kDa and the accession number is Q6DFY2. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q6DFY2 |
别名 | opioid binding protein/cell adhesion molecule-like, C230027C17, 3732419F12, Obcam, B930023M13Rik, 2900075O15Rik, Gm181, AI844366 |
蛋白构建 | Mouse OPCML (NP_808574.2) (Ala5-Val313) |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 32.7 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Opioid-binding Cell Adhesion Molecule (OBCAM), also known as OPCML, is a GPI-anchored cell adhesion molecule in the plasma membrane. This neuron-specific protein consists of three immunoglobulin (Ig)-like domains anchored to the membrane through a glycosylphosphatidylinositol (GPI)-tail. OPCML also belongs to the member of the IgLON family, a subgroup of the immunoglobulin superfamily, consisting of three members, LAMP, OBCAM, and Neurotrimin. These molecules interact homophilically and heterophilically within the family, and OBCAM acts only as heterodimers with LAMP or Neurotrimin and possibly inhibits neurite outgrowth from cerebellar granule cells. The OBCAM has been presumed to play a role as a cell adhesion/recognition molecule. Furthermore, the OPCML protein defects may play an important role in the carcinogenesis of cervical or ovarian cancers, and this gene is regarded as a candidate TSG (tumor suppressor gene). |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
OPCML Protein, Mouse, Recombinant (His) AI-844366 opioid binding protein/cell adhesion molecule-like C230027C17 3732419F12 Obcam AI 844366 B930023M13Rik 2900075O15Rik Gm181 AI844366 recombinant recombinant-proteins proteins protein