生物活性

产品描述	NPR1 Protein, Human, Recombinant (mFc) is expressed in HEK293 Cells. The accession number is NP_000897.3.
生物活性	Immobilized Human NPR1, mFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 43.2 ng/ml determined by ELISA (QC Test).
研究背景	NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
种属	Human
表达系统	HEK293 Cells
标签	C-mFc
蛋白编号	NP_0008973
蛋白构建	Gly33-Glu473
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	74.56 kDa (Predicted); 80-110 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

NPR1 Protein, Human, Recombinant (mFc)

NPR1 Protein, Human, Recombinant (mFc)

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