生物活性

产品描述	NPR1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is NP_000897.3.
生物活性	Immobilized Biotinylated Human NPR1, His-Avi Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8 ng/ml determined by ELISA (QC Test). Anti-NPR1 Ab.2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
研究背景	NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	NP_0008973
蛋白构建	Gly33-Glu473
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	51.8 kDa (Predicted); 65-80 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

NPR1 Protein, Human, Recombinant (His & Avi), Biotinylated

NPR1 Protein, Human, Recombinant (His & Avi), Biotinylated

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