Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 56.5 kDa and the accession number is P46531.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,180 | 5日内发货 | ||
500 μg | ¥ 20,700 | 5日内发货 | ||
1 mg | ¥ 34,600 | 5日内发货 |
生物活性 | Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 μM as determined in SPR assay. |
产品描述 | Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 56.5 kDa and the accession number is P46531. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P46531 |
别名 | lin-12, Mis6, N1, 9930111A19Rik, Notch-1, TAN1, NOTCH1 |
蛋白构建 | Ala19-Gln526 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 56.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
Notch 1 Protein, Human, Recombinant (His & Avi), Biotinylated lin-12 Mis6 N1 N 1 9930111A19Rik N-1 Notch-1 NOTCH 1 TAN1 TAN 1 TAN-1 NOTCH1 recombinant recombinant-proteins proteins protein