Noggin/NOG Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 23.1 kDa and the accession number is Q13253.
规格 | 价格/CNY | 货期 | 数量 | |
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20 μg | ¥ 1,750 | 现货 | ||
100 μg | ¥ 4,430 | 5日内发货 | ||
200 μg | ¥ 7,550 | 5日内发货 | ||
500 μg | ¥ 15,210 | 5日内发货 |
生物活性 |
Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells. The ED50 for this effect is 10-80 ng/mL.
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产品描述 | Noggin/NOG Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 23.1 kDa and the accession number is Q13253. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | Tag Free |
蛋白编号 | Q13253 |
别名 | noggin, SYNS1, SYM1 |
蛋白构建 | Human NOG (NP_005441.1) (Met1-Cys232) |
蛋白纯度 |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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分子量 | 23.1 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7 and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both types I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, the formation of joints in the skeletal system, and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion. |
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Noggin/NOG Protein, Human, Recombinant noggin SYM-1 SYNS-1 SYNS1 SYM1 SYNS 1 SYM 1 recombinant recombinant-proteins proteins protein