Mucin-1/MUC1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 41 kDa and the accession number is P15941-11.
生物活性 | Testing in progress |
产品描述 | Mucin-1/MUC1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 41 kDa and the accession number is P15941-11. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-mFc |
蛋白编号 | P15941-11 |
别名 | mucin 1, cell surface associated, ADMCKD1, EMA, MCKD1, Mucin 1, MUC-1, MCD, MCKD, ADMCKD, PUM, CA15-3, MUC-1/X, KL-6, MAM6, MUC-1/SEC, PEM, MUC1/ZD, H23AG, PEMT, CD227 |
蛋白构建 | The Human MUC1 isoform 2 (NP_001018016.1) extracellular domain (Met1-Gly167) was fused with the Fc region of Mouse IgG1 at the C-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 41 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Mucin 1, cell surface-associated (MUC1) or polymorphic epithelial mucin (PEM) is a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach, and pancreas. MUC-1/CC1/CD227 Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized, and recycled to the cell membrane. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. The alpha subunit has cell adhesive properties. MUC-1/CC1/CD227 Can act both as an adhesion and an anti-adhesion protein. This protein May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. MUC-1/CC1/CD227 participated in modulates signaling in ERK, SRC, and NF-kappa-B pathways. In the activated T-cells, MUC-1/CC1/CD227 influences directly or indirectly the Ras/MAPK pathway. MUC-1/CC1/CD227 Promotes tumor progression and regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Mucin-1/MUC1 Protein, Human, Recombinant (mFc) mucin 1, cell surface associated ADMCKD1 EMA MCKD1 Mucin 1 KL 6 MUC-1 MAM-6 MUC1 MCD MUC 1 MCKD ADMCKD PUM CA15-3 MUC-1/X ADMCKD 1 KL-6 MAM6 CD-227 MUC-1/SEC PEM MCKD-1 CD 227 KL6 ADMCKD-1 MUC1/ZD H23AG PEMT MAM 6 MCKD 1 CD227 recombinant recombinant-proteins proteins protein