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Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc)

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货号 TMPH-00419

别名 Microbial collagenase, Collagenase ColA, colA, 120 kDa collagenase

Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen. Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 39.7 kDa and the accession number is P43153.

Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc)
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Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc)

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货号 TMPH-00419 别名 Microbial collagenase, Collagenase ColA, colA, 120 kDa collagenase

Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen. Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 39.7 kDa and the accession number is P43153.

规格价格库存数量
5 μg
¥ 825
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10 μg
¥ 1,360
20日内发货
20 μg
¥ 2,290
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50 μg
¥ 3,460
20日内发货
100 μg
¥ 5,360
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200 μg
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1 mg
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产品介绍

生物活性
产品描述
Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen. Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 39.7 kDa and the accession number is P43153.
生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景
Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen.
种属
C.perfringens
表达系统
E. coli
标签N-10xHis, C-Myc
蛋白编号P43153
氨基酸序列NKIYTFDELNRMNYSDLVELIKTISYENVPDLFNFNDGSYTFFSNRDRVQAIIYGLEDSGRTYTADDDKGIPTLVEFLRAGYYLGFYNKQLSYLNTPQLKNECLPAMKAIQYNSNFRLGTKAQDGVVEALGRLIGNASADPEVINNCIYVLSDFKDNIDKYGSNYSKGNAVFNLMKGIDYYTNSVIYNTKGYDAKNTEFYNRIDPYMERLESLCTIGDKLNNDNAWLVNNALYYTGRMGKFREDPSISQRALERAMKEYPYLSYQYIEAANDLDLNFGGKN
蛋白构建90-370 aa
蛋白纯度
> 85% as determined by SDS-PAGE.
Microbial collagenase Protein, Clostridium perfringens, Recombinant (His & Myc)
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名Microbial collagenase, Collagenase ColA, colA, 120 kDa collagenase
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量39.7 kDa (predicted)
颜色White
物理性状Lyophilized powder
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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剂量转换

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关键词

120 kDa-collagenase
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