生物活性

产品描述	MDL-1/CLEC5A Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q9NY25-1.
生物活性	Immobilized Biotinylated Human MDL-1, His Avi Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA (QC Test).
研究背景	In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
种属	Human
表达系统	HEK293 Cells
标签	N-His-Avi
蛋白编号	Q9NY25-1
蛋白构建	Pro28-Lys188
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	21.3 kDa (Predicted); 43-53 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

MDL-1/CLEC5A Protein, Human, Recombinant (His & Avi), Biotinylated

MDL-1/CLEC5A Protein, Human, Recombinant (His & Avi), Biotinylated

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