生物活性

产品描述	MAdCAM-1 Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is XP_045235080.2.
生物活性	Immobilized Biotinylated Cynomolgus MADCAM1, His Avi Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.1 ng/ml determined by ELISA (QC Test).
研究背景	Mucosal addressin cell adhesion molecule–1 (MAdCAM-1) is an endothelial cell marker that is recognized by its ligands on lymphocytes: L-selectin and α4β7 integrin. MAdCAM-1 is highly expressed on intestinal endothelium and has, recently, been shown to play an important role in the development of acute GVHD in animal model.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	XP_0452350802
蛋白构建	Gln22-Gln295
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	31.95 kDa (Predicted); 60-70 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

MAdCAM-1 Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

MAdCAM-1 Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

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